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Catalog Number | orb2315028 |
---|---|
Category | Antibodies |
Description | Recombinant CD137 / 4-1BB / TNFRSF9 Antibody |
Target | TNFRSF9 |
Clonality | Monoclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Concentration | Purified Ab with BSA and Azide at 200ug/ml |
Immunogen | Recombinant fragment (around aa 19-188) of human CD137 protein (exact sequence is proprietary) |
UniProt ID | Q07011 |
MW | 32kDa (monomer); 85kDa (dimer) |
Tested applications | ELISA, FC, IF, IHC, WB |
Dilution range | ELISA (For coating, order antibody without BSA);,Immunofluorescence (1-2ug/ml); Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
Application notes | Positive Control: HEK293 cells, Jurkat whole cell lysate or recombinant human CD137 protein (WB). Human kidney cancer, liver, stomach or thyroid cancer tissue (IHC-P). Expressed on the surface of activated T-cells. |
Antibody Type | Recombinant Antibody |
Clone Number | 4-1BB/4552R |
Expression System | CHO cells |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | 4-1BB Ligand Receptor T Cell; Antigen 4-1BB Homolo Read more... |
Note | For research use only |
Entrez | 3604 |
ELISA, FC, IF, IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |