Recombinant Cadherin 2 Antibody / CDH2 / N-Cadherin

• Catalog Number: orb2635368
• Category: Antibodies
• Description: Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325, Cadherin 2 and CDH2. CDH2 is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CDH2 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, the protein has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CDH2 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CDH2/7070R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant Cadherin 2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Cadherin 2 antibody is available for research use only.
• UniProt ID: P19022
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Cadherin 2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human heart tissue with recombinant Cadherin 2 antibody (clone CDH2/7070R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human heart tissue with recombinant Cadherin 2 antibody (clone CDH2/7070R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Cadherin 2 antibody (clone CDH2/7070R) as confirmation of integrity and purity.