Reactive Oxygen Species (ROS) Detection Fluorometric Assay Kit

SKU: orb1173196

Description

Reactive Oxygen Species (ROS) Detection Assay Kit provides a detection method based on the fluorescent probe DCFH-DA. DCFH-DA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS).

Images & Validation

• Application Notes:
  Reactive Oxygen Species (ROS) Detection Assay Kit provides a simple, sensitive, rapid ROS detection method. ROS detection is based on the fluorescent probe DCFH-DA . DCFH-DA is a cell-permeable sensitive probe used to detect intracellular reactive oxygen species (ROS). This probe could be hydrolyzed into DCFH by esterase in the cell while passing through the living cell membrane. DCFH is no fluorescence and cannot transmit the cell membrane, which then can be could be oxidated by ROS and produce the fluorescent DCF. Then the fluorescent could be detect by flow cytometry or fluorescence microscopy , and according which the level of cellular reactive oxygen species can be analyzed.

Storage & Handling

• Storage: Please store the whole kit at -20°C upon receipt ,and protect from light. The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only