RCAN1 Antibody

• Catalog Number: orb1267324
• Category: Antibodies
• Description: RCAN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This RCAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-68 amino acids from the N-terminal region of human RCAN1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 28 kDa
• Target: RCAN1
• UniProt ID: P53805
• NCBI: P53805
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Calcipressin-1, Adapt78, Down syndrome critical re
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in 293 cell line lysates (35 ug/lane).

Western blot analysis of RCAN1 using rabbit polyclonal RCAN1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCAN1 gene.

Fluorescent confocal image of A431 cell stained with RCAN1 Antibody (N-term).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RCAN1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RCAN1 immunoreactivity is localized to Cytoplasm significantly.