RBP4, RBP1, RBP2 Antibody

• Catalog Number: orb1251893
• Category: Antibodies
• Description: RBP4, RBP1, RBP2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM442
• Tested applications: IF, IHC
• Reactivity: Goat, Human, Monkey, Mouse, Rabbit, Rat
• Isotype: IgG1, kappa
• Immunogen: Retinol binding protein purified from human plasma was used as the immunogen for this RBP antibody. Its epitope localizes between amino acids 74-182 of human RBP.
• Concentration: 0.2 mg/mL
• Dilution range: Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the RBP antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RBP4, RBP1, RBP2
• UniProt ID: P02753, P50120, P09455
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the RBP antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with RBP (SPM442)