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Catalog Number | orb251543 |
---|---|
Category | Antibodies |
Description | RBP4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human RBP4 recombinant protein (Position: E19-201L). Human RBP4 shares 86% amino acid (aa) sequence identity with both mouse and rat RBP4. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, - Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat ELISA , 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 23010 MW |
UniProt ID | P02753 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Retinol-binding protein 4;Plasma retinol-binding p Read more... |
Note | For research use only |
Application notes | WB: The detection limit for RBP4 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis using Anti-RBP4 Picoband antibody.Lane 1:Rat Liver Tissue;2:Mouse Liver Tissue;3:HEPG2 Cell;4:COLO320 Cell.
IHC analysis of RBP4 using anti-RBP4 antibody.RBP4 was detected in paraffin-embedded section of human liver cancer tissues.
IHC analysis of RBP4 using anti-RBP4 antibody.RBP4 was detected in paraffin-embedded section of human liver cancer tissues.
IHC analysis of RBP4 using anti-RBP4 antibody.RBP4 was detected in paraffin-embedded section of mouse kidney tissues.
IHC analysis of RBP4 using anti-RBP4 antibody.RBP4 was detected in paraffin-embedded section of mouse kidney tissues.
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