RBM3 antibody

• Catalog Number: orb20194
• Category: Antibodies
• Description: Goat polyclonal antibody to RBM3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:2000, WB: 0.01-0.03 μg/ml
• Conjugation: Unconjugated
• MW: 17.2
• Target: RBM3
• Entrez: 5935
• Protein Sequence: DRYSGGNYRDNYDN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti RBM3 antibody, anti RNA binding motif (RNP1,
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx.17kDa band observed in lysates of cell line HepG2 (calculated MW of 17.2kDa according to NP_006734.1). Recommended concentration: 0.01-0.03 μg/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb20194 (0.01ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.