RbBP5 Antibody

• Catalog Number: orb1530678
• Category: Antibodies
• Description: RbBP5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 500 and C-term
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:250 - 1:1,000; ChIP - 1 - 3 µg as per Dou et al., Nat Struct Mol Biol 13 (8):713-719, 2006. Previous lots of this antibody have performed in this application.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RbBP5
• UniProt ID: Q15291
• NCBI: NP_005048.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: SWD1; RBQ3; SWD1, Set1c WD40 repeat protein, homol
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human RbBP5 by western blot.Samples: Whole cell lysate from HeLa, NCI-H460, HEK293T, Malme-3M, and Jurkat cells. Antibody: Affinity purified rabbit anti-RbBP5 antibody at 0.04 µg/ml.

Detection of mouse RbBP5 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-RbBP5 antibody at 0.04 µg/ml.

Detection of human RbBP5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human RbBP5 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-RbBP5 antibody.

ChIP-chip scatter plot of anti-RbBP5 enriched DNA binding sites versus input reference DNA.

Localization of RbBP5 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-RbBP5 antibody and analyzed by DNA sequencing.