Rat Vasoactive Intestinal Peptide (VIP) ELISA Kit

SKU: orb1806855

Description

Rat Vasoactive Intestinal Peptide (VIP) ELISA Kit

Images & Validation

• Application Notes:
  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat VIP. During the reaction, Rat VIP in the sample or standard competes with a fixed amount of Rat VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat VIP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat VIP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples
• Assay Type: Competitive
• Assay Time: 1.5H
• Range: 7.81-500pg/mL
• Sensitivity: 4.69 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

PHM27

Quick Database Links

Gene Symbol

VIP

UniProt

P01283