Rat Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit

• Catalog Number: orb2668031
• Category: Assays and Kits
• Description: Rat Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit
• UniProt ID: Q02445
• Application notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TFPI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TFPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TFPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TFPI in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.61 ng/mL
• Sample Types: plasma, urine, tissue homogenates, cell culture supernates and other biological fluids
• Alternative names: TFI; EPI; LACI; TFPI1; Lipoprotein-Associated Coag
• Note: For research use only