Rat PCSK9 Protein

• Catalog Number: orb257742
• Category: Proteins
• Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
• Reactivity: Rat
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 13.9 kDa and 59.3 kDa
• Target: PCSK9
• Protein Sequence: NP_954862.2
• Source: Rat PCSK9, His Tag (orb257742) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 691 (Accession # P59996-1).
• Expression System: HEK293
• Expression Region: Gln 31 - Gln 691
• Endotoxins: 1.0 EU per μg
• NCBI: NP_954862.2
• Storage: -20℃
• Buffer/Preservatives: 50 mM Tris, 150 mM NaCl, pH7.5
• Alternative names: PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 59.3 kDa respectively. The protein migrates as 16 kDa and 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Rat PCSK9 protein

Line graph illustrates about the sensitivity range of Rat PCSK9 protein using different concentrations