Rat MBL1 ELISA Kit

• Catalog Number: orb1173622
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose-binding protein A(MBL1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mannose-binding protein A(MBL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose-binding protein A(MBL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose-binding protein A(MBL1) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Tested applications: ELISA
• Reactivity: Rat
• Range: 0.16-10 ng/mL
• UniProt ID: P19999
• Solubility (25°C): 10 ng/mL
• Sensitivity: 0.059 ng/mL
• Alternative names: MBL1,Mbl1Mannose-binding protein A, MBP-A, Mannan-
• Note: For research use only
• Application notes: Sandwich
• Sample Types: Serum, plasma and other biological fluids
• Expiration Date: Please enquire.