Rat Interleukin 6 (IL6) ELISA Kit

SKU: orb775023

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6(IL6).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL6 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 3.3 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MGI2-A ELISA Kit, MGI2A ELISA Kit, HGF ELISA Kit, BSF2 ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, B-Cell Stimulatory Factor-2 ELISA Kit, Hybridoma/Plasmacytoma Growth Factor ELISA Kit, Hepatocyte Stimulating Factor ELISA Kit, Cytotoxic T-Cell Differentiation Factor ELISA Kit

Quick Database Links

UniProt

P20607

References

Z Sun, HY Ren, JY Song Tong-luo Qu-tong Plaster relieves chronic muscle pain by reducing inflammation via NCF1-CXCL10-ICAM1 axis in SD rats Tradit Med Res, (2025)

PubMed:

Applications

ELISA

Reactivity

Rat