Rat IL1 beta protein

• Catalog Number: orb80052
• Category: Proteins
• Description: Rat IL1 beta protein
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 95.0% as determined by SDS-PAGE.
• Conjugation: Unconjugated
• Target: IL1 beta
• Solubility (25°C): It is recommended to reconstitute the lyophilized Interleukin 1b in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: VPIRQLHCRL RDEQQKCLVL SDPCELKALH LNGQNISQQV VFSMSFVQGE TSNDKIPVAL GLKGLNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKTKV EFESAQFPNW YISTSQAEHR PVFLGNSNGR DIVDFTMEPV SS.
• Biological Activity: The ED50 range= 0.2-0.5 ng/ml, determined by the dose dependent proliferation of mouse D10S cells.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The protein was lyophilized without any additives.
• Alternative names: IL 1 Proteins, IL1 beta Proteins, IL-1 beta Protei
• Note: For research use only
• Application notes: Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.558 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-1b as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Rat IL1 beta protein