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Rat IL1 beta protein
Catalog Number: orb80052
| Catalog Number | orb80052 |
|---|---|
| Category | Proteins |
| Description | Rat IL1 beta protein |
| Tested applications | FA, HPLC, SDS-PAGE |
| Purity | > 95.0% as determined by SDS-PAGE. |
| Conjugation | Unconjugated |
| Target | IL1 beta |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin 1b in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | VPIRQLHCRL RDEQQKCLVL SDPCELKALH LNGQNISQQV VFSMSFVQGE TSNDKIPVAL GLKGLNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKTKV EFESAQFPNW YISTSQAEHR PVFLGNSNGR DIVDFTMEPV SS. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 range= 0.2-0.5 ng/ml, determined by the dose dependent proliferation of mouse D10S cells. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | The protein was lyophilized without any additives. |
| Alternative names | IL 1 Proteins, IL1 beta Proteins, IL-1 beta Protei Read more... |
| Note | For research use only |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.558 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-1b as Reference Standard. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Rat IL1 beta protein
Rat IL-1b protein [orb633357]
ELISA, WB
Greater than 95% by SDS-PAGE gel analyses
17.3 KDa
E.Coli
1 mg, 200 μg, 100 μg, 50 μgRat IL1B Protein, His Tag [orb2698624]
> 98%, determined by SDS-PAGE
This protein contains the mature form of rat IL1B (Q63264-1) (Val 117-Ser 268) was expressed from E. coli.
20 μg, 5 μgRat IL1B Protein, His Tag [orb2698625]
> 90%, determined by SDS-PAGE
This protein contains the pro form of rat IL1B (Q63264-1) (Met 1-Ser 268) was fused with a polyhistidine tag at the N-terminus and expressed from E. coli.
50 μg, 20 μg
