Rat IL-1Beta ELISA Kit

• Catalog Number: orb1173314
• Category: Assays and Kits
• Description: Rat IL-1β ELISA Kit employs a two-site sandwich ELISA to quantitate Rat IL-1β.
• Tested applications: ELISA
• Reactivity: Rat
• Tag: IL1B
• Entrez: 24494
• UniProt ID: Q5BKB0
• Storage: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
• Alternative names: IL1B, IL-1, IL1-BETA, IL1F2, catabolin, preinterle
• Note: For research use only
• Application notes: Rat IL-1β ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1β in samples. An antibody specific for IL-1β has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1β present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1β is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Sample Types: Cell culture supernatants#Serum#Plasma#Other biological fluids
• Expiration Date: Please enquire.