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Catalog Number | orb343809 |
---|---|
Category | Proteins |
Description | Rat IgG2a Kappa isotype control Antibody |
Tested applications | ELISA, FC, FLISA, IF, SDS-PAGE, WB |
Isotype | IgG2a |
Concentration | 1 mg/ml |
Dilution range | ELISA: User Optimized, FLISA: User Optimized, FC: 1:1000-1:5000, IF: User Optimized, WB: User Optimized |
Form/Appearance | Liquid (sterile filtered) |
Purity | RAT IgG2a Kappa isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG1 and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG1a, IgG1b, IgG3, IgM, and IgA. Light and heavy chain composition has been confirmed by SDS-PAGE. |
Conjugation | Unconjugated |
Source | Rat |
Biological Origin | Rat |
Storage | Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rat Isotype Control, Rat IgG2a isotype, Rat IgG2a Read more... |
Note | For research use only |
Application notes | RAT IgG2a Kappa isotype control has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user. |
Expiration Date | 12 months from date of receipt. |
FC, IF | |
Rat | |
Monoclonal | |
PE |
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