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Rat IgG2a Isotype Control PE
Catalog Number: orb623586
| Catalog Number | orb623586 |
|---|---|
| Category | Antibodies |
| Description | Rat monoclonal isotype control against Rat IgG2a conjugated to PE |
| Clonality | Monoclonal |
| Isotype | Rat IgG2a |
| Conjugation | PE |
| Concentration | 0.1 mg/ml |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Tested applications | FC |
| Application notes | Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background. |
| Antibody Type | Secondary Antibody |
| Clone Number | RTG2A1-1 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Note | For research use only |
Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using rat IgG2a Isotype control (RTG2A1-1) FITC antibody (concentration in sample 15 μg/ml).
