Rat IgG1 Kappa isotype control Antibody

SKU: orb343807

Description

Rat IgG1 Kappa isotype control Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: FC, SDS-PAGE
• Dilution Range: FC: 1:1000-1:5000
• Application Notes:
  RAT IgG1 Kappa isotype control has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

Key Properties

• Isotype: IgG1
• Clone No.: RG1K
• Purity: RAT IgG1 Kappa isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG1 and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Light and heavy chain composition has been confirmed by SDS-PAGE.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rat Isotype Control, Rat IgG1 isotype, Rat IgG1 kappa isotype