Rat IgG1 isotype control Phycoerythrin Antibody

• Catalog Number: orb343824
• Category: Proteins
• Description: Rat IgG1 isotype control Phycoerythrin Antibody
• Tested applications: DOT, ELISA, FC
• Isotype: IgG1
• Concentration: 0.5 mg/mL
• Dilution range: ELISA: 1:2000 - 1:20,000, FC: 1:1000-1:5000
• Form/Appearance: Lyophilized
• Purity: RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Phycoerythrin Conjugated Rat IgG1 confirmed by Dot Blot.
• Conjugation: RPE
• Source: Rat
• Biological Origin: Rat
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rat Isotype Control, Rat IgG1 PE isotype, RAT IgG1
• Note: For research use only
• Application notes: RAT IgG1 isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
• Expiration Date: 12 months from date of receipt.

Dot Blot results of Rat IgG1 isotype control Phycoerythrin Conjugated. Antigen: Rat IgG1 isotype control RPE Conjugated. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT.