Rat IgG (H&L) antibody (ATTO 594)

• Catalog Number: orb347827
• Category: Antibodies
• Description: Rat IgG (H&L) antibody (ATTO 594)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FLISA, IF, WB
• Reactivity: Rat
• Isotype: IgG
• Immunogen: Rat IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: >1:20,000, IF: >1:5,000, WB: >1:10,000
• Form/Appearance: Lyophilized
• Purity: Rat IgG (H&L) Antibody ATTO 594 was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.
• Conjugation: ATTO-594
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat anti-Rat IgG ATTO594 Conjugated Antibody, Goa
• Note: For research use only
• Application notes: Anti-Rat IgG (H&L) conjugated to ATTO 594 has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of human umbilical vein endothelial cells using Rat IgG (H&L) antibody (ATTO 594)