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Catalog Number | orb347882 |
---|---|
Category | Antibodies |
Description | Rat IgG (H&L) antibody (Alkaline Phosphatase) |
Species/Host | Sheep |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Rat |
Isotype | IgG |
Immunogen | Rat IgG whole molecule |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Rat IgG and Rat Serum. |
Conjugation | AP |
Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Sheep Anti-Rat IgG alkaline phosphatase Conjugated Read more... |
Note | For research use only |
Application notes | Anti-Rat IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
ELISA, ICC, IHC, WB | |
Rat | |
Goat | |
Polyclonal | |
AP |
ELISA, IHC, WB | |
Rabbit | |
Rat | |
Polyclonal | |
AP |
DOT, ELISA, IHC, WB | |
Rat | |
Goat | |
Polyclonal | |
AP |
ELISA, IHC, WB | |
Rat | |
Goat | |
Polyclonal | |
AP |
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