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    Rat IgG F(ab')2 antibody (Peroxidase)

    Rat IgG F(ab')2 antibody (Peroxidase)

    Catalog Number: orb347860

    DispatchUsually dispatched within 5-10 working days
    $ 463.00
    Catalog Numberorb347860
    CategoryAntibodies
    DescriptionRat IgG F(ab')2 antibody (Peroxidase)
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityRat
    IsotypeIgG
    ImmunogenRat IgG F(ab')2 fragment
    Concentration2.0 mg/mL
    Dilution rangeELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
    Form/AppearanceLyophilized
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against Rat IgG F(c).
    ConjugationHRP
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Alternative namesRabbit Anti-Rat IgG F(ab')2 peroxidase Conjugated
    Read more...
    NoteFor research use only
    Application notesThis product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:3,000 of the reconstitution concentration is suggested for this product.
    Expiration Date12 months from date of receipt.
    • Rat IgG F(ab')2 Peroxidase Antibody [orb346349]

      HRP

      This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.

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      ELISA,  IHC,  WB

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      ELISA,  IHC,  WB

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