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Catalog Number | orb80047 |
---|---|
Category | Proteins |
Description | Insulin-Like Growth Factor Rat Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7687 Dalton. IGF-I is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | rIGF1 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IGF1 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Biological Origin | E.coli |
Biological Activity | The ED50, was calculated methods1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be 30 ng/ml.2. Type IGF receptor binding assay ED50 was found to be < 10 ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The protein was lyophilized with no additives. |
Alternative names | Somatomedin C protein, IGF-I protein, IGFIA protei Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Rat IGF1 protein
98.00% | |
12 KDa (reducing condition) |
98.00% | |
34.7 kDa (predicted) |
Greater than 98.0% as determined by SDS-PAGE. | |
Escherichia Coli |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
9.6 kDa | |
E.Coli |