Rat Glu(Glutamic Acid) ELISA Kit

• Catalog Number: orb1736530
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Glu protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Glu. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Glu in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Rat
• Range: 156.25-10000 ng/mL
• Sensitivity: 48.2 ng/mL
• Alternative names: Glutamate
• Note: For research use only
• Sample Types: biological fluids
• Expiration Date: Please enquire.