Rat Enolase, Neuron Specific (NSE) ELISA Kit

SKU: orb776453

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enolase, Neuron Specific(NSE).

Research Area

Cancer Biology, Infectious Disease & Virology, Neuroscience, Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NSE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NSE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NSE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NSE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rat
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.232 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ENO2 ELISA Kit, Enolase 2 ELISA Kit, Gamma Enolase ELISA Kit, 2-phospho-D-glycerate hydro-lyase ELISA Kit, Neural enolase ELISA Kit

Quick Database Links

UniProt

P07323