Rat Cys-C(Cystatin C) ELISA Kit

• Catalog Number: orb1146703
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin C(Cys-C). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cystatin C(Cys-C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystatin C(Cys-C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cystatin C(Cys-C) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Rat
• Range: 0.16-10 ng/mL
• UniProt ID: P14841
• Sensitivity: 0.10 ng/mL
• Protein Length: 3.5h
• Alternative names: CST3, Cystatin 3, ARMD11, Post-Gamma-Globulin
• Note: For research use only
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.