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Catalog Number | orb1088215 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat CTN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CTN in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Rat |
Range | 3.13-200 µg/mL |
Sensitivity | 0.89 µg/mL |
Protein Length | 2h |
Alternative names | Creatinine Read more... |
Note | For research use only |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
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