Rat PD-L1

SKU: orb2994074

Description

Recombinant Rat Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Ala18-Thr238 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Rat
• Biological Activity: BLI: Loaded Rat PD-L1-His(Cat#orb2994074) on HIS1K Biosensor, can bind Anti-Human PDL1 mAb-Fc(Cat#orb2993144) with an affinity constant of 0.90 nM as determined in BLI assay. (Regularly tested) Immobilized Anti-Human PDL1 mAb-Fc(Cat#orb2993144) at 8μg/ml (100 μl/well) can bind Rat PD-L1-His(Cat#orb2994074).The ED50 of Rat PD-L1-His(Cat#orb2994074) is 0.28 ug/ml.
• Tag: C-6His
• Molecular Weight: Predicted: 26.1 KDa. Observed: 40-60 KDa, reducing conditions
• Expression Region: Ala18-Thr238
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

Quick Database Links

UniProt

D4AE25