You have no items in your shopping cart.
Cart summary
Item 1 of 1
| Catalog Number | orb778961 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Alpha-Melanocyte Stimulating Hormone(aMSH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-Melanocyte Stimulating Hormone(aMSH). |
| Reactivity | Rat |
| Concentration | 10000 pg/mL |
| Tested applications | ELISA |
| Application notes | standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat aMSH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat aMSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat aMSH in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 156.25-10000 pg/mL |
| Sensitivity | 47.6 pg/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | α-MSH ELISA Kit, Intermedins ELISA Kit, Alpha-Mela Read more... |
| Note | For research use only |
- Item 1 of 1
