Rat 5HTR2A(5-Hydroxytryptamine Receptor 2A) ELISA Kit

• Catalog Number: orb1146868
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxytryptamine Receptor 2A(5HTR2A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptamine Receptor 2A(5HTR2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Hydroxytryptamine Receptor 2A(5HTR2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine Receptor 2A(5HTR2A) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Rat
• Range: 0.16-10 ng/mL
• UniProt ID: P14842
• Sensitivity: 0.065 ng/mL
• Protein Length: 3.5h
• Alternative names: 5-HT2A, HTR2, Serotonin Receptor 2A
• Note: For research use only
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.