RARA antibody

• Catalog Number: orb18965
• Category: Antibodies
• Description: Goat polyclonal antibody to RARA
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:8000, WB: 0.5-2 μg/ml
• Conjugation: Unconjugated
• MW: 50.8; 50.8; 39.7
• Target: RARA
• Entrez: 5914
• Protein Sequence: SPSLSPSSHRSSPATQSP
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti RARA antibody, anti RAR antibody, anti NR1B1
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Cerebellum shows staining of Purkinje cytoplasm. Recommended concentration, 4-6ug/ml.WB: Approx 50kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 50.8kDa according to NP_000955.1; NP_001019980.1). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18965 (0.05ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb18965 (4ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.