RAR gamma Rabbit Polyclonal Antibody

SKU: orb101688

Description

RAR gamma Rabbit Polyclonal Antibody

Research Area

DNA and RNA Research, Dermatology, Zinc Finger Protein

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Retinoic Acid Receptor gamma (151-250/454aa)
• Target: RARG
• Molecular Weight: 50 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NR1B3; Nuclear receptor subfamily 1 group B member 3; RARC; RARG; Retinoic acid receptor gamma 1; Retinoic acid receptor gamma 2; Retinoic acid receptor gamma; Retinoic acid receptor gamma polypeptide; Retinoic acid receptor, gamma; RARG_HUMAN.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (RAR gamma) polyclonal Antibody, Unconjugated (orb101688) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: 293T (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, Raji (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-RAR gamma (orb101688) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 55 kD.

Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human HeLa cell lysates, Lane 3: Human A549 cell lysates, Primary: Anti-RAR gamma (orb101688) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kDa, Observed band size: 48 kDa.

Quick Database Links

Gene Symbol

RARG

UniProt

P13631