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Catalog Number | orb548411 |
---|---|
Category | Antibodies |
Description | RAP80 Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | ADCH-21 |
Tested applications | ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | A synthesized peptide derived from human RAP80 Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Dilution range | WB 1:1000-1:5000IHC 1:50-1:200ICC/IF 1:50-1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
UniProt ID | Q96RL1 |
Sensitivity | < 50pg/ml |
Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of UIMC1 expression in 293T cell lysate using anti-UIMC1 antibody (orb548411). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UIMC1 antigen affinity purified polyclonal antibody (Catalog # orb548411) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for UIMC1.
ICC, IHC-Fr, IHC-P, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
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