RAP1A monoclonal antibody (M01), clone 3E1

• Catalog Number: orb2292279
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant RAP1A.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3E1
• Tested applications: ELISA, PLA, WB
• Reactivity: Human
• Isotype: IgG2a Kappa
• Immunogen: RAP1A (AAH14086.1, 1 a.a. ~ 184 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL
• NCBI: AAH14086.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged RAP1A is approximately 1 ng/ml as a capture antibody.

Proximity Ligation Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot detection against Immunogen (45.98 KDa).