RANGAP1 Antibody

• Catalog Number: orb1247902
• Category: Antibodies
• Description: RANGAP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: ASEDIAKLAETLAK-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in 3T3 lysates (predicted MW of 68kDa according to NP_002874). Recommended for use at 0.1-0.5ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration: 2-4ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RANGAP1
• UniProt ID: P46060
• NCBI: NP_002874.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RANGAP1, KIAA1835, Ran GTPase activating protein 1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in 3T3 lysates (predicted MW of 68kDa according to NP_002874). Recommended for use at 0.1-0.5ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration: 2-4ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247902 staining (0.2 ug/ml) of 3T3 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1247902 (2.5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.