RALBP1 Antibody, KO Validated

• Catalog Number: orb1274538
• Category: Antibodies
• Description: RALBP1 Antibody, KO Validated
• Target: RALBP1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 396-655 of human RALBP1 (NP_006779.1).
• UniProt ID: Q15311
• MW: Observed: 100kDa
• Tested applications: IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RalA-binding protein 1, RalBP1, 76 kDa Ral-interac
• Note: For research use only

Western blot analysis of extracts of various cell lines, using RALBP1 antibody (orb1274538) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and RALBP1 knockout (KO) HeLa cells, using RALBP1 antibody (orb1274538) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat spleen using RALBP1 antibody (orb1274538) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using RALBP1 antibody (orb1274538) at dilution of 1:100 (40x lens).