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Catalog Number | orb334527 |
---|---|
Category | Antibodies |
Description | RALB Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, WB |
Predicted Reactivity | Bovine, Monkey |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human RALB (166-203aa DKVFFDLMREIRTKKMSENKDKNGKKSSKNKKSFKERC), different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 23409 MW |
UniProt ID | P11234 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Ras-related protein Ral-B;RALB; Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of Jurkat cells using anti-RALB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of RALB using anti-RALB antibody.Lane 1:Rat Brain Tissue;2:Rat Thymus Tissue;3:Rat Lung Tissue;4:Mouse Spleen Tissue;5:Mouse Liver Tissue;6:HELA Cell;7:22RV1 Cell.
IF analysis of RALB using anti-RALB antibody. RALB was detected in immunocytochemical section of A549 cells.
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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