Raf-B (phospho-Thr753) antibody

• Catalog Number: orb770153
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Raf-B (phospho-Thr753)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human B-Raf around the phosphorylation site of Thr753. AA range:717-766
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 84
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-BRAF antibody, anti-BRAF1 antibody, anti-RAFB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using B-Raf (Phospho-Thr753) Antibody.

Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30', using B-Raf (Phospho-Thr753) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).