RadChemDosi Cell Survival Assay Kit

• Catalog Number: orb342313
• Category: Assays and Kits
• Description: RadChemDosi Cell Survival Assay Kit
• Conjugation: Unconjugated
• Storage: Store Kit at 2-8° C prior to opening. See kit insert for complete instructions.
• Alternative names: Radiation dosing assay, Gamma radiation assay, dos
• Note: For research use only
• Application notes: RadChemDosi Cell Survival Assay Kit contains enough reagents for 100 test if 1 ml is used for irradiation. RadChemDosi is sensitive to measure the dose rate as low as 2.5Gy/min at various positions in a Cesium irradiator chamber. In addition to calibrating the dose rate at each position in a radiation chamber periodically, it can also be used to measure the dose rate in each well of a 6 and 24 wells plate.
• Expiration Date: Please enquire.

RadChemDosi™ Cell Survival Assay to measure gamma radiation dose rate by producing free radicals that reacts with biomolecules in mammalian and other types of cells. y = 0.043x + 0.000 r2 = 0.998; y = 0.014x + 0.003 r2 = 0.990; y = 0.010x + 0.004 r2 = 0.945. Dose rate with turn table on: Position 1- 1193.82 rads/min or 11.92 Gy/min. Position 2- 388.36 rads/min or 3.88 Gy/min. Position 3- 277.4 rads/min or 2.774 Gy/min.

RadChemDosi™ is an effective way to measure dose rate for ionizing radiation. In addition to calibrating the dose rate at each position in a radiation chamber periodically, it can also be used to measure the dose rate in each well of a 6 and 24 wells plate. Measurment of gamma dose rate with RadChemDosi™ in 6 well plates. Dose Rate with the turntable on: Position 2- 360.28 rads/min or 3.60 Gy/min for each well. y = 0.013x + 0.000 r2 = 1.000 (each).

RadChemDosi™ is sensitive to measure the dose rate as low as 2.5 Gy/min at various positions in a Cesium irradiator chamber. The variation in radiation dose rate measured in each of the six well plate in a radiation machine with no turntable suggests that the dose rate calibration by RadDose assay is required to accurately measure the dose given to cells in each well of a six well plate. The calibration by RadDose is essential in radiation chambers without turntable. y = 0.047x + 0.015 r2 = 0.970; y = 0.072x + 0.022 r2 = 0.972; y = 0.040x + 0.013 r2 = 0.977; y = 0.033x - 0.003 r2 = 0.980; y = 0.028x + 0.002 r2 = 0.999; y = 0.031x - 0.001 r2 = 0.971.