RAD23B Antibody

• Catalog Number: orb1526861
• Category: Antibodies
• Description: RAD23B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: between 359 and 409
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RAD23B
• UniProt ID: P54727
• NCBI: NP_002865.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: XP-C repair complementing complex 58 kDa; XP-C rep
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse RAD23B by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-RAD23B antibody orb1526861 used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of rat RAD23B by western blot. Samples: Whole cell lysate (50 µg) from C6 cells. Antibodies: Affinity purified rabbit anti-RAD23B antibody orb1526861 used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human and mouse RAD23B by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RAD23B antibody orb1526861 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 10 µg/mg lysate. RAD23B was also immunoprecipitated by rabbit anti-RAD23B antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human RAD23B by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RAD23B (Cat. No. orb1526861) used at a dilution of 1: 1, 000 (0.2 µg/ml). Detection: DAB