RACGAP1 antibody

• Catalog Number: orb18360
• Category: Antibodies
• Description: Goat polyclonal antibody to RACGAP1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Bovine, Canine, Human
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 1 μg/ml
• Conjugation: Unconjugated
• MW: 71.0; 63.9; 72.3; 61.9
• Target: RACGAP1 / MgcRacGAP
• Entrez: 29127
• Protein Sequence: GRQGNFFASPMLK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti RACGAP1 antibody, anti MgcRacGAP antibody, an
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration, 1ug/ml.WB: Approx 60kDa band observed in Human Testis lysates (calculated MW of 71.0kDa according to NP_037409.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Toure et al., J Biol Chem 1998 Mar 13;273(11):6019-23; PMID: 9497316). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Testis lysate using RACGAP1 antibody

Immunohistochemical staining of Human Testis using RACGAP1 antibody

Western blot analysis of HEK293 lysate using RACGAP1 antibody

Immunohistochemical staining of Human Testis using RACGAP1 antibody

Flow cytometric analysis of MCF7 cells using RACGAP1 antibody

Immunofluorescence analysis of MCF7 cells using RACGAP1 antibody