Rac1 C protein

• Catalog Number: orb533576
• Category: Proteins
• Description: Rho family GTPases Rac1 and Cdc42 belong to the Ras superfamily of small GTP-binding proteins. Rac1 is involved in regulation of a variety of signal transduction processes, ranging from cell growth to cy-toskeletal organisation required for cell motility and cell adhesion. It has been shown that Rac1 is inhibited by 6-thioguanine triphosphate (6-Thio-GTP), a metabolite generated by the immunosuppressive and anti-inflammatory drug Azathioprine. In intestinal epithelial cells the pro-inflammatory mediator Leukotriene D 4induces Rac-dependent migration via 10-fold increase of Rac activation. The C-terminal deletion of five amino acids of Rac1ΔC (aa 1-187) includes arginine 188 which is part of the polybasic domain consisting of six contiguous basic amino acids. Deletion or mutation of the polybasic residues decrease the intrinsic GTPase activity and result in a loss of the self-stimulatory GAP activity. Protein preparation is 100% GDP-loaded, measured by HPLC.
• Clonality: Recombinant
• Form/Appearance: liquid (Supplied in 30 mM Tris-HCl pH 7.0 and 5 mM DTE)
• Purity: > 90% (SDS-PAGE)
• MW: Theoretical MW: 20.9 kDa (187 amino acids)
• Source: E. coli
• Storage: store at -80 °C
• Note: For research use only
• Expiration Date: 6 months from date of receipt.