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Catalog Number | orb630471 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to RAC1 |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | ELISA, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG1 |
Immunogen | ras-related C3 botulinum toxin substrate 1(rho family, small GTP binding protein Rac1) |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000-1:4000; IP: 1:200-1:2000; IHC: 1:50-1:500; IF: 1:50-1:500 |
Form/Appearance | liquid |
Purity | ≥95% as determined by SDS-PAGE |
Conjugation | Unconjugated |
MW | 21KD |
Target | RAC1 |
UniProt ID | P63000 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
HSCT6 cells were subjected to SDS PAGE followed by western blot with orb630471 (Rac1 antibody) at dilution of 1:2000.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using orb630471 (Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
IP Result of anti-RAC1 (IP: orb630471, 3 ug; Detection: orb630471 1:700) with HL-60 cells lysate 4000 ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using orb630471 (RAC1 Antibody) at dilution of 1:400.
IF, IHC-Fr, IHC-P | |
Canine, Equine, Gallus, Porcine, Rabbit | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Canine, Mouse, Rabbit | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |