Rabbit sC5b-9 ELISA Kit

• Catalog Number: orb1173569
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble Terminal Complement Complex C5b-9(sC5b-9). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble Terminal Complement Complex C5b-9(sC5b-9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble Terminal Complement Complex C5b-9(sC5b-9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Soluble Terminal Complement Complex C5b-9(sC5b-9) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Tested applications: ELISA
• Reactivity: Rabbit
• Range: 0.16-10 ng/mL
• Sensitivity: 0.10 ng/mL
• Protein Length: 3.5h
• Alternative names: MAC, Membrane Attack Complex, C5b9, TCC C5b-9
• Note: For research use only
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.