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Catalog Number | orb347680 |
---|---|
Category | Antibodies |
Description | Rabbit IgG F(ab')2 antibody (Peroxidase) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Rabbit |
Isotype | IgG |
Immunogen | Anti-Rabbit IgG F(ab')2 fragment was produced by repeated immunization with Rabbit IgG F(ab')2 fragment in goat. |
Concentration | 2.0 mg/mL |
Dilution range | ELISA: 1:130,000, IHC: 1:1,000 - 1:10,000, WB: 1:2,000 - 1:20,000 |
Form/Appearance | Lyophilized |
Purity | Anti-RABBIT IgG F(ab')2 (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c) or Human Serum Proteins. |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | Goat Anti-Rabbit IgG F(ab')2 Antibody peroxidase C Read more... |
Note | For research use only |
Application notes | Anti-Rabbit IgG F(ab')2 fragment has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit IgG, anti-Rabbit IgG F(ab’)2 and anti-Rabbit Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin. | |
Rabbit |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rabbit IgG, anti-Rabbit IgG F(ab’)2 and anti-Rabbit Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin. | |
Rabbit |
ELISA, IHC, WB | |
Feline | |
Rabbit | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Feline | |
Rabbit | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Gallus | |
Rabbit | |
Polyclonal | |
HRP |
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