Rabbit Human Ig Kappa Light Chain (free) antibody

• Catalog Number: orb22351
• Category: Antibodies
• Description: Precipitating polyclonal rabbit antiserum to free human Ig kappa chain. To detect and identify free light chain type and Bence Jones protein in serum or urine by immunoelectrophoresis or double radial immunodiffusion. It is of great importance as an aid in the diagnosis of B cell malignancies such a multiple myeloma and non-Hodgkin lymphoma, and in monitoring their therapy. In immunoelectrophoresis use 1.2 ul of sample in a well with a diameter of 1 mm against 60 ul antiserum in a 20 mm long channel. Electrophoresis for 1 hour, 20 mA. The detection limit of this technique in serum is about 25 mg/l; in the case 100 times concentrated urine is used the detection limit can be brought down to 0.5 mg/l. The fact that in about 40% of the case the Bence Jones protein was only detectable in either serum or urine emphasizes the necessity to investigate both fluids.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Form/Appearance: Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity.
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Source: A pool of purified Bence Jones kappa proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Storage: Storage: The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product
• Buffer/Preservatives: Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.