Rabbit GAPDH ELISA Kit

• Catalog Number: orb1173551
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Tested applications: ELISA
• Reactivity: Rabbit
• Range: 1.57-100 ng/mL
• UniProt ID: P46406
• Sensitivity: 0.56 ng/mL
• Protein Length: 3.5h
• Alternative names: G3PD, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH
• Note: For research use only
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.