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Catalog Number | orb1567041 |
---|---|
Category | Tools |
Description | Rabbit Buffy Coat Preparation |
Concentration | n/a |
Form/Appearance | Frozen Cell Pellet |
Purity | Buffy coat preparations are composed of leukocytes, trace amounts of red blood cells and other components. Buffy coat preparations are separated after a single low-speed centrifugation to remove the platelet rich plasma, buffy coat layer and erythrocytes (RBC). If desired, you may further purify this preparation of leukocytes by sedimentation using Percoll gradient centrifugation. |
Conjugation | Unconjugated |
Source | Rabbit |
Biological Origin | Rabbit |
Storage | Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles. |
Buffer/Preservatives | None. See application note. |
Alternative names | Rabbit Buffy Coat Preparation Read more... |
Note | For research use only |
Application notes | Buffy coat preparations are suitable for use as a source of nucleated PBMC. Buffy coat preparations are separated after a single low-speed centrifugation to remove the platelet rich plasma, buffy coat layer and erythrocytes (RBC). Buffy coat preparations are generally enriched for leukocytes (WBC) and contain trace amounts of red cells and other blood components. If desired, you may further purify this preparation of leukocytes by sedimentation using Percoll gradient centrifugation. This product is prepared in a buffer composed of 20.5 mg/ml Dextrose, 4.2mg/ml Sodium Chloride, 8.0 mg/ml Citric Acid Tri-sodium, adjusted to pH 6.1 |
Expiration Date | 12 months from date of receipt. |
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