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Catalog Number | orb1527754 |
---|---|
Category | Antibodies |
Description | ZCCHC8 Antibody |
Target | ZCCHC8 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Whole IgG |
Concentration | 1000 µg/ml |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Immunogen | Between 1 and 50 |
UniProt ID | Q6NZY4 |
Tested applications | IHC, IP |
Dilution range | WB - Not recommended. Use rabbit anti-ZCCHC8 antibody A301-806A.; IP - 2 - 5 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:1,000 - 1:5,000 |
Application notes | Format: Whole IgG |
Antibody Type | Primary Antibody |
Storage | 2 - 8°C |
Alternative names | TRAMP-like complex RNA-binding factor ZCCHC8; zinc Read more... |
Note | For research use only |
NCBI | NP_060082.2 |
Detection of human ZCCHC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-ZCCHC8 antibody orb1527754 used for IP at 3 µg/mg lysate.
Detection of human ZCCHC8 by immunohistochemistry.Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZCCHC8 (Cat. No. orb1527754) used at a dilution of 1: 5, 000 (0.2µg/ml) and 1: 2, 000 (0.5µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100.