Rabbit anti-WWOX Antibody

• Catalog Number: orb1523643
• Category: Antibodies
• Description: WWOX Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human, Mouse
• Immunogen: Between 75 and 125
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. A304-078A has not performed satisfactorily when used for WB of WWOX in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of WWOX.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: WWOX
• UniProt ID: Q9NZC7
• NCBI: P_057457.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: PRO0128; WW domain-containing oxidoreductase; WOX1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human WWOX by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody orb1523643 used for IP at 6 µg/mg lysate.

Detection of human WWOX by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-WWOX used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Detection of mouse WWOX by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-WWOX used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB